Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
Demonstration of Infectious Bovine Rhinotracheitis Virus Antigen by Immunoperoxidase Method in Tissues of Aborted Bovine Fetuses Preserved for 25 Years in Paraffin Blocks
Hiroaki OGINOSumio INUIMinoru NARITA
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1996 Volume 58 Issue 5 Pages 459-460

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Abstract

Infectious bovine rhinotracheitis (IBR) virus antigen was demonstrated by the immunoperoxidase method in tissues of two aborted bovine fetuses, which had been stored for 25 years after fixing in formalin and embedding in paraffin. Necrotic foci were detected in the liver, kidney. adrenal gland, and thymus of the fetuses. Coincidenting with the distribution of the necrotic foci, IBR virus antigen was demonstrated by immunostaining. The present study proved the immunoperoxidase method in one of useful techniques to demonstrate IBR virus antigen in tissue sections from preserved paraffin blocks.

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© The Japanese Society of Veterinary Science
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