Elsevier

Methods in Enzymology

Volume 148, 1987, Pages 350-382
Methods in Enzymology

[34] Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes

https://doi.org/10.1016/0076-6879(87)48036-1Get rights and content

Publisher Summary

This chapter presents detailed information on chlorophylls and carotenoids to give practical directions toward their quantitative isolation and determination in extracts from leaves, chloroplasts, thylakoid particles, and pigment proteins. The chapter focuses on the spectral characteristics and absorption coefficients of chlorophylls, pheophytins, and carotenoids, which are the basis for establishing equations to quantitatively determine them. Therefore, the specific absorption coefficients of the pigments are re-evaluated. This is achieved by using a two-beam spectrophotometer of the new generation, which allows programmed automatic recording and printing out of the proper wavelengths and absorbancy values. Several procedures have been developed for the separation of the photosynthetic pigments, including column (CC), paper (PC), and thin-layer chromatography (TLC) and high-pressure liquid chromatography (HPLC). All chloroplast carotenoids exhibit a typical absorption spectrum that is characterized by three absorption maxima (violaxanthin, neoxanthin) or two maxima with one shoulder (lutein and β-carotene) in the blue spectral region.

References (59)

  • T. Watanabe et al.

    FEBS Lett.

    (1985)
  • H.K. Lichtenthaler et al.

    Biochim. Biophys. Acta

    (1981)
  • J.M. Anderson et al.

    FEBS Lett.

    (1978)
  • J. Argyroudi-Akoyunoglou et al.

    FEBS Lett.

    (1979)
  • D. Siefermann-Harms et al.

    FEBS Lett.

    (1979)
  • T. Omata et al.

    Biochim. Biophys. Acta

    (1984)
  • T.-Y. Leong et al.

    Biochim. Biophys. Acta

    (1983)
  • C. Critchley

    Biochim. Biophys. Acta

    (1985)
  • K. Eskins et al.

    J. Chromatogr.

    (1977)
  • T. Braumann et al.

    J. Chromatogr.

    (1979)
  • T. Watanabe et al.

    Biochim. Biophys. Acta

    (1985)
  • G. Mackinney

    J. Biol. Chem.

    (1941)
  • J.F.G.M. Wintermans et al.

    Biochim. Biophys. Acta

    (1965)
  • V.V. Klimov et al.

    FEBS Lett.

    (1977)
  • T. Omata et al.

    Biochim. Biophys. Acta

    (1984)
  • T.W. Goodwin
  • H.K. Lichtenthaler
  • R. Willstätter et al.

    Untersuchungen über Chlorophyll

    (1913)
  • A. Seybold et al.

    Planta

    (1970)
  • H.K. Lichtenthaler et al.

    Photosynth. Res.

    (1981)
  • H.K. Lichtenthaler et al.

    Z. Naturforsch., C: Biosci.

    (1982)
  • H. H. Strain, B. T. Cope, and W. A. Svec, this series, Vol. 23, p....
  • A. Hager
  • D. Siefermann-Harms
  • K. Grumbach

    Z. Naturforsch., C: Biosci.

    (1983)
  • H.A.W. Schneider

    Ber. Dtsch. Bot. Ges.

    (1975)
  • W. Rüdiger et al.
  • B.H. Davies
  • J.P. Markwell et al.
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