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Genetic variability and inter species relationship between wild and cultivated yams (Dioscorea spp.) from Koraput, India based on molecular and morphological markers

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Abstract

Wild yams (Dioscorea spp.) are important tuber crops used both as vegetable and medicine by the tribal people of Koraput, India. There is deficiency of documented information on genetic structure and diversity of wild yams and its genetic assessment is necessary for crop improvement program. The present study assessed the level of genetic diversity of eight wild and one cultivated yam species of Koraput by using different morphological and molecular markers. Significant variation in different yield and morphological traits was observed among the studied yam species. The major morphological traits such as branch number, stem thickness, tuber depth, tuber length, number of tubers per plant and yield showed high genetic heritability accompanied with high genetic advance and major determinants of phenotypic diversity. Molecular profiling was carried out by taking five simple sequence repeat markers. A total of 10 polymorphic bands with an average of two were detected at the loci of the five markers across the nine yam species. Genetic similarity analysis revealed that some wild yam species such as D. oppositifolia, D. hamiltonii and D. pubera showed higher genetic similarity with cultivated (D. alata) species. The knowledge of the extent of genetic variations of wild yam species is important for planning of the genetic conservation and the utilization of this resource especially for genetic improvement.

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Acknowledgements

Authors are grateful to the Head, Department of Biodiversity and Conservation of Natural Resources for providing necessary facilities for the work and also grateful to University Grant Commission (UGC) (Grant No. 14/CUO/PHD/NONNET/01), New Delhi, India for providing Non-NET Fellowship.

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Authors and Affiliations

  1. Department of Biodiversity and Conservation of Natural Resources, Central University of Orissa, Koraput, Odisha, 764021, India

    Bandana Padhan & Debabrata Panda

  2. National Rice Research Institute (ICAR), Cuttack, Odisha, 753006, India

    Arup K. Mukherjee & Sangram K. Mohanty

  3. TERI, Deakin NanoBiotechnology Centre, The Energy and Resources Institute, Gurugram, Haryana, 122001, India

    Sangram K. Lenka

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  1. Bandana Padhan
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  2. Arup K. Mukherjee
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  3. Sangram K. Mohanty
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  4. Sangram K. Lenka
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  5. Debabrata Panda
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Contributions

BP and DP designed the experiments, cultivated the plants and performed the measurement of morphological traits. BP, AKM and SKM performed the molecular analysis. SKL and DP analyzed the data and wrote the paper. All authors read and provided helpful discussions for the manuscript.

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Correspondence to Debabrata Panda.

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Padhan, B., Mukherjee, A.K., Mohanty, S.K. et al. Genetic variability and inter species relationship between wild and cultivated yams (Dioscorea spp.) from Koraput, India based on molecular and morphological markers. Physiol Mol Biol Plants 25, 1225–1233 (2019). https://doi.org/10.1007/s12298-019-00691-3

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  • DOI: https://doi.org/10.1007/s12298-019-00691-3

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