Skip to main content
SpringerLink
Log in

Electrochemical glucose biosensor by electrostatic binding of PQQ-glucose dehydrogenase onto self-assembled monolayers on gold

  • Original Paper
  • Published:
Journal of Applied Electrochemistry Aims and scope Submit manuscript

Abstract

Efficient binding of enzymes onto the electrode surface has been prerequisite for the construction of sensitive biosensors and biochips. Here, a simple and robust construction of electrochemical glucose biosensor based on pyrroloquinoline quinone-glucose dehydrogenase was demonstrated. The glucose biosensor was fabricated by binding the enzyme onto the anionic self-assembled monolayers on gold electrode via electrostatic interactions. The resulting glucose biosensor gave rise to twofold higher detection sensitivity than that by covalent conjugation under the same condition. Surface plasmon resonance and atomic force microscopy analyses revealed that electrostatic binding of the enzyme leads to much higher surface density of the enzyme. This approach will find wide applications to the development of robust enzyme-based biosensors and biochips.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Wang J (2008) Chem Rev 108:814

    Article  CAS  Google Scholar 

  2. Goswami P, Sarma AK, Vatsyayan P, Minteer SD (2009) Biosens Bioelectron 24:2313

    Article  Google Scholar 

  3. Duine JA, Frank J (1981) Trends Biochem Sci 6:278

    Article  CAS  Google Scholar 

  4. Duine JA (1999) J Biosci Bioeng 88:231

    Article  CAS  Google Scholar 

  5. Sode K, Ootera T, Shirahane M, Witarto AB, Igarashi S, Yoshida H (2000) Enzyme Microbiol Technol 26:491

    Article  CAS  Google Scholar 

  6. Yamazaki T, Kojima K, Sode K (2000) Anal Chem 72:4689

    Article  CAS  Google Scholar 

  7. Kano K, Tsujimura S, Ishii A, Abo T (2008) J Electroanal Chem 614:67

    Article  Google Scholar 

  8. Zayats M, Katz E, Baron R, Willner I (2005) J Am Chem Soc 127:12400

    Article  CAS  Google Scholar 

  9. Raj CR, Jena BK (2006) Anal Chem 78:6332

    Article  Google Scholar 

  10. Yehezkeli O, Tel-Vered R, Reichlin S, Willner I (2011) ACS Nano 5:2385

    Article  CAS  Google Scholar 

  11. Mano N, Flexer V, Durand F, Tsujimura S (2011) Anal Chem 83:5721

    Article  Google Scholar 

  12. Ikebukuro K, Kohiki Y, Sode K (2002) Biosens Bioelectron 17:1075

    Article  CAS  Google Scholar 

  13. El-Shora HM, Youssef MM (2008) J Appl Sci Res 4:545

    CAS  Google Scholar 

  14. Kricka LJ (1994) Clin Chem 40:347

    CAS  Google Scholar 

  15. Decher G (1997) Science 277:1232

    Article  CAS  Google Scholar 

  16. Kim HS, Yoon HC (2000) Anal Chem 72:922

    Article  Google Scholar 

  17. Mrksich M, Whitesides GM (1996) Annu Rev Biophys Biomol 25:55

    Article  CAS  Google Scholar 

  18. Patel N, Davies MC, Hartshorne M, Heaton RJ, Roberts CJ, Tendler SJB, Williams PM (1997) Langmuir 13:6485

    Article  CAS  Google Scholar 

  19. Jiang SY, Li LY, Chen SF (2003) Langmuir 19:2974

    Article  Google Scholar 

  20. Nakano K, Doi K, Tamura K, Katsumi Y, Tazaki M (2003) Chem Commun 13:1544

    Article  Google Scholar 

  21. Gooding JJ, Hibbert DB (1999) Trac Trend Anal Chem 18:525

    Article  CAS  Google Scholar 

  22. Guiomar AJ, Guthrie JT, Evans SD (1999) Langmuir 15:1198

    Article  CAS  Google Scholar 

  23. Jin W, Bier F, Wollenberger U, Scheller F (1995) Biosens Bioelectron 10:823

    Article  CAS  Google Scholar 

  24. Dijkstra BW, Oubrie A, Rozeboom HJ (1999) Proc Natl Acad Sci USA 96:11787

    Article  Google Scholar 

  25. Ryabov AD, Razumiene J, Meskys R, Gureviciene V, Laurinavicius V, Reshetova MD (2000) Electrochem Commun 2:307

    Article  Google Scholar 

  26. Razumiene J, Gureviciene V, Vilkanauskyte A, Marcinkeviciene L, Bachmatova I, Meskys R, Laurinavicius V (2003) Sens Actuator B Chem 95:378

    Article  Google Scholar 

  27. Tanaka S, Igarashi S, Ferri S, Koji S (2005) BMC Biochem 6:1

    Article  Google Scholar 

  28. Durand F, Stines-Chaumeil C, Flexer V, Andre I, Mano N (2010) Biochem Biophys Res Commun 402:750

    Article  CAS  Google Scholar 

  29. Kluger R, Alagic A (2004) Bioorg Chem 32:451

    Article  CAS  Google Scholar 

  30. Available from: http://wwwbiacorecom/homelasso

  31. Dijkstra BW, Oubrie A, Rozeboom HJ, Kalk KH, Olsthoorn AJJ, Duine JA (1999) EMBO J 18:5187

    Article  Google Scholar 

  32. Lahiri J, Isaacs L, Tien J, Whitesides GM (1999) Anal Chem 71:777

    Article  CAS  Google Scholar 

  33. Hong MY, Yoon HC, Kim HS (2003) Langmuir 19:416

    Article  CAS  Google Scholar 

  34. Roth CM, Sader JE, Lenhoff AM (1998) J Colloid Interface Sci 203:218

    Article  CAS  Google Scholar 

  35. Kim YP, Hong MY, Kim J, Oh E, Shon HK, Moon DW, Kim HS, Lee TG (2007) Anal Chem 79:1377

    Article  CAS  Google Scholar 

  36. Ivanova EP, Wright JP, Pham DK, Brack N, Pigram P, Alekseeva YV, Demyashev GM, Nicolau DV (2006) Biomed Mater 1:24

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the National Research Foundation of Korea (NRF) (K21003001810-11E0100-01510), and Basic Science Research Program through the NRF (2011-0022757), Pioneer Research Program for Converging Technology (20090081537), Advanced Biomass R&D Center (ABC) (ABC-2010-0029800) of Ministry of Education, Science and Technology (MEST) of Korea, and the Seoul R&D Program (10919).

Author information

Authors and Affiliations

  1. Department of Life Science, Hanyang University, Seoul, 133-791, Korea

    Young-Pil Kim

  2. Research Institute for Natural Sciences, Hanyang University, Seoul, 133-791, Korea

    Young-Pil Kim

  3. Department of Biological Sciences, KAIST, Daejeon, 305-701, Korea

    Sang Jin Park & Hak-Sung Kim

  4. Korea Institute of Industrial Technology, Cheonan, 331-825, Korea

    Dohoon Lee

Authors
  1. Young-Pil Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sang Jin Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dohoon Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hak-Sung Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Young-Pil Kim or Hak-Sung Kim.

Additional information

Young-Pil Kim and Sang Jin Park have contributed equally to this study.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, YP., Park, S.J., Lee, D. et al. Electrochemical glucose biosensor by electrostatic binding of PQQ-glucose dehydrogenase onto self-assembled monolayers on gold. J Appl Electrochem 42, 383–390 (2012). https://doi.org/10.1007/s10800-012-0409-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10800-012-0409-1

Keywords

Search

Navigation