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Multifarious activity of bioformulated Pseudomonas fluorescens PS1 and biocontrol of Sclerotinia sclerotiorum in Indian rapeseed (Brassica campestris L.)

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Abstract

PGPR strain Pseudomonas fluorescens PS1 was evaluated to formulate carrier based bioformulations. The viability of P. fluorescens PS1 was monitored at different time intervals during the period of storage at room temperature in different carriers such as soil, charcoal, sawdust and sawdust-soil. Sawdust-soil was found to be the most efficient carrier material for P. fluorescens PS1 followed by other carriers. After 1 year of storage, P. fluorescens PS1 was re-isolated and assayed for its antifungal activity against Sclerotinia sclerotiorum a phytopathogenic fungus causing stem blight in Indian mustard, Brassica campestris. Results of scanning electron microscopy exhibited that P. fluorescens PS1 caused morphological alteration in mycelia of S. sclerotiorum as evident by hyphal perforation, and fragmented lysis. Seed bacterization of B. campestris with P. fluorescens PS1 induced enhanced seed germination, increased overall plant growth as well as reduced stem blight in mustard with improved yield. These findings demonstrate that P. fluorescens PS1 has significant potential to raise disease-free crops due to the presence of a wide array of PGP characteristics.

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Acknowledgements

DKM thanks CSIR—(TMOP & M), New Delhi, UGC, New Delhi and UCOST, Dehradun for providing financial support.

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Authors and Affiliations

  1. Department of Botany and Microbiology, Gurukul Kangri University, Haridwar, 249404, Uttrakhand, India

    Abhinav Aeron, R. C. Dubey & D. K. Maheshwari

  2. S. B. S. P. G. Institute of Biomedical Sciences and Research, Balawala, Dehradun, 48161, Uttarakhand, India

    Piyush Pandey

  3. School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, 712-749, Republic of Korea

    Vivek K. Bajpai

  4. Department of Biotechnology, Daegu University, Gyeongsan, Gyeongbuk, 712-749, Republic of Korea

    Sun Chul Kang

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  1. Abhinav Aeron
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  2. R. C. Dubey
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  4. Piyush Pandey
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Correspondence to Sun Chul Kang.

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Aeron, A., Dubey, R.C., Maheshwari, D.K. et al. Multifarious activity of bioformulated Pseudomonas fluorescens PS1 and biocontrol of Sclerotinia sclerotiorum in Indian rapeseed (Brassica campestris L.). Eur J Plant Pathol 131, 81–93 (2011). https://doi.org/10.1007/s10658-011-9789-z

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  • DOI: https://doi.org/10.1007/s10658-011-9789-z

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