Abstract
To obtain further information about the formation of cellulose Iα and Iβ, cross polarization/magic angle spinning (CP/MAS)13C NMR spectroscopy was used to study the effects of polymeric additives, stirring and culture temperature on the Iα When xyloglucan (XG) or carboxymethyl cellulose sodium salt (CMC) was added to the incubation medium, the amount of cellulose Iα decreased markedly, from a normal level of 64% to as low as 30%, with the most additive giving the lowest levels of Iα. Moreover, stirring causes mixtures containing even small amounts of XG to have a large effect. These results suggest that CMC or XG interferes with the aggregation of fibrillar units into the normal ribbon assemblies. It may be that there is a strain associated with this aggregation that results in the higher-energy Iα form. Thus, cellulose Iβ may grow preferentially when the strain caused by aggregation is not present. Lower temperatures (36–10 °C) gave an increase in Iα (from 56 to 72%).
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Atalla, R. H. and VanderHart, D. L. (1984)Science 223, 283–285.
Atalla, R. H., Gast, J. C., Sindorf, D. W., Bartuska, V. J. and Maciel, G. E. (1980)J. Amer. Chem, Soc. 102, 3249–3251.
Atalla, R. H., Hackney, J. M., Uhlin, I. and Thompson, N. S. (1993)Int. J. Biol. Macromol. 15, 109–112.
Chanzy, H., Imada, K., Mollard, A., Vuong, R. and Barnard, F. (1979)Protoplasma 100, 317.
Chanzy, H., Henrissat, B., Vincendon, M., Tanner, S. F. and Belton, P. S. (1987)Carbohydrate Res. 160, 1–11.
Earl, W. L. and VanderHart, D. L. (1980)J. Amer. Chem. Soc. 102, 3251–3252.
Gardner, K. H. and Blackwell, J. (1974)Biopolymers 13, 1975–2001.
Haigier, C. H. (1991) InByosynthesis and Biodegradation of Cellulose (C. H. Haigler and P. J. Weimer, eds.). New York-Basel-Hong Kong: Marcel Dekker, pp. 99–124.
Haigier, C. H. and Benziman, M. (1982) InCellulose and Other Natural Polymer Systems. Biogenesis, Structure and Degradation (R. M. Brown, Jr., ed.). New York-London: Plenum Press, pp. 273–297.
Haigler, C. H., White, A. R., Brown, R. M. Jr. and Cooper, K. M. (1982)J. Cell Biol. 94, 64–69.
Hayashi, T. (1989) InPlant Fibers. Measuring β-Glucan Deposition in Plant Cell Walls (H. F. Linskens and J. F. Jackson, eds.). Berlin-Heidelberg-New York-London-Paris-Tokyo: Springer-Verlag, pp. 138–160.
Hayashi, T., Marsden, M. P. F. and Delmer, D. P. (1987)Plant Physiol. 83, 384–389.
Hirai, A., Horii, F. and Kitamaru, R. (1985)Bull. Inst. Chem. Res., Kyoto University 63, 340–359.
Idem (1987)Macromolecules 20, 1440–1442.
Horii, F. (1989) inNuclear Magnetic Resonance in Agriculture (P. E. Pfeffer and W. V. Gerasimowicz, eds.). Boca Raton, FL: CRC Press, Chapter 10, pp. 311–335.
Horii, F., Hirai, A. and Kitamaru, R. (1982)Polymer Bull. (Berlin) 8, 163–170.
Idem (1987a)Macromolecules 20, 2117–2120.
Horii, F., Yamamoto, H., Hirai, A., Kitamaru, R., Tanahashi, M. and Higuchi, T. (1987b)ibid., 2946–2949.
Horii, F., Yamamoto, H. Hirai, A. and Kitamaru, R. (1990) InCellulose: Structural and Functional Aspects (J. K. Kennedy, G. O. Phillips and P. A. Williams, eds.). Chichester, UK: Ellis Horwood, pp. 125–130.
Kai, A., Ogusuri, J. and Kobayashi, Y. (1982)Nihon Kagakukaishi 8, 1394–1399.
Sarko, A. and Muggli, R. (1974)Macromolecules 7, 486–494.
Sugiyama, J., Okano, T., Yamamoto, H. and Horii, F. (1990)ibid. 23, 3196–3198.
Sugiyama, J., Vuong, R. and Chanzy, H. (1991)ibid. 24, 4168–4175.
Torchia, D. A. (1978)J. Mag. Res. 30, 613–616.
VanderHart, D. L. and Atalla, R. H. (1984)Macromolecules 17, 1465–1472.
Yamamoto, H. and Horii, F. (1993)ibid. 26, 1313–1317.
Yamamoto, H., Horii, F. and Odani, H. (1989)ibid. 22, 4130–4132.
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Yamamoto, H., Horn, F. In Situ crystallization of bacterial cellulose I. Influences of polymeric additives, stirring and temperature on the formation celluloses I α and I β as revealed by cross polarization/magic angle spinning (CP/MAS)13C NMR spectroscopy. Cellulose 1, 57–66 (1994). https://doi.org/10.1007/BF00818798
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DOI: https://doi.org/10.1007/BF00818798