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The effect of age, gender, and time between blood draw and start of centrifugation on the size outcomes of platelet-rich fibrin (PRF) membranes

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Abstract

Objectives

Platelet-rich fibrin (PRF) has been utilized in regenerative dentistry as a supra-physiological concentrate of autologous growth factors capable of stimulating tissue regeneration. Due to the variability in the macroscopic morphology/size of PRF membranes observed between patients, we were interested in studying the effects of patient age, gender, and time between blood draw and the start of centrifugation on the size outcomes of PRF membranes. Despite PRF therapy being increasingly more popular in private practice, to date, no study has investigated the effects of the delay between blood draw and the start of centrifugation in a clinical setting.

Materials and methods

A total of 60 patients enrolled in this study were divided into 6 groups of 10 patients each, including male and female patients categorized into age groups 21–40, 41–60, and 61–80 years. From each patient, a total of five PRF membranes were fabricated from 10-mL tubes following centrifugation starting after 0, 30, 60, 90, and 120 s. In total, 300 PRF membranes were produced in this study to investigate the effects of patient age, gender, and time on the size outcomes of PRF membranes.

Results

A longer delay by the clinician before starting centrifugation following blood draw led to a smaller final size of PRF membranes. At 90 s following blood draw, a significant (13%) reduction in PRF membrane size was observed. After 120 s, a significant (23%) reduction was observed. Additionally, female patients had on average 17% larger membranes compared to men (p < 0.05, 300 samples). Lastly, the size outcomes of the PRF membranes was largest in patients aged 61–80, followed by those aged 41–60 and 21–40. However, no statistically significant differences in PRF membrane sizes were reported between age groups.

Conclusions

The time at which a centrifugation procedure begins following blood draw is critical to optimize the size outcomes of PRF membranes. In general, approximately 15 s is required per tube to harvest 9–10 cc of blood. Therefore, a 60- to 90-s interval between blood draw and the start of centrifugation should be a parameter that is respected by clinicians to avoid significant changes in the macroscopic morphology/size of fabricated PRF membranes. Furthermore, females and older patients produced larger membranes, likely due to lower red blood cell counts derived from their peripheral blood.

Clinical relevance

The findings from the present study demonstrate that on average, a clinician has approximately 60–90 s between blood draw and the start of the centrifugation cycle to produce standard-sized PRF membranes. Shortly thereafter, a significant reduction in size is observed. Additionally, females and older patients were found to produce larger PRF membranes. Centrifugation protocols may therefore be adapted accordingly.

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Author information

Author notes

  1. Richard J. Miron and Anika Dham contributed equally to this work.

Authors and Affiliations

  1. Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland

    Richard J. Miron & Anton Sculean

  2. The Miron Research Lab, Fort Lauderdale, FL, USA

    Richard J. Miron, Anika Dham & Uttma Dham

  3. Department of Oral Implantology, University of Wuhan, Wuhan, China

    Yufeng Zhang

  4. The Pikos Institute, Trinity, FL, USA

    Michael A. Pikos

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  1. Richard J. Miron
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Correspondence to Richard J. Miron.

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The authors declare that they have no conflict of interest.

Ethical approval

No ethical approval was required for this study, as human samples were not identified.

Informed consent

For this type of study, informed consent was provided prior to blood draw to conduct the outlined experiments.

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Miron, R.J., Dham, A., Dham, U. et al. The effect of age, gender, and time between blood draw and start of centrifugation on the size outcomes of platelet-rich fibrin (PRF) membranes. Clin Oral Invest 23, 2179–2185 (2019). https://doi.org/10.1007/s00784-018-2673-x

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  • DOI: https://doi.org/10.1007/s00784-018-2673-x

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