1 Materials Research 2006 Vol: 9(2):. DOI: 10.1590/S1516-14392006000200012

Pulp response of anionic lyophilized collagen matrix with or without hydroxyapatite after pulpotomy in dog's teeth

The aim of the present study was to evaluate histologically the pulp response of anionic lyophilized collagen matrix with or without hydroxyapatite as a biomaterial pulp-capping agent in pulpotomy of dogs' teeth. Sixty pre-molar roots from three dogs were used. After pulpotomy, the remaining pulp tissue was capped with one of the following materials: Group I (20 roots): anionic lyophilized collagen matrix; Group II (20 roots): anionic lyophilized collagen matrix associated with hydroxyapatite; Group III (10 roots): calcium hydroxide (p.a.) paste in saline; Group IV (10 roots): zinc oxide eugenol cement. After 90 days the animals were killed by anesthetic overdose and the teeth were removed and submitted to histological processing. According to the histopathological results, we concluded that the zinc oxide eugenol cement and anionic lyophilized collagen matrix with or without hydroxyapatite did not present satisfactory pulp response and that calcium hydroxide is the suitable material for pulpotomy.

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References
  1. Assed S, Leonardo MR, Silva LAB, Nelson-Filho P, Pinto DMG. Calcium hydroxide pulpotomy in young permanent teeth with periapical involvement. Brazilian Endodontic Journal. 2(1) , 38-42 (1997) .
  2. Ranly DM. Pulpotomy therapy in primary teeth: new modalities for old rationales. Pediatric Dentistry. 16(6) , 403-409 (1994) .
  3. Leonardo MR, Silva LAB, Leonardo RD, Utrilla LS, Assed S. Histological evaluation of therapy using a calcium hydroxide dressing for teeth with incompletely formed apices and periapical lesions. Journal of Endodontics. 19(7) , 348-352 (1993) .
  4. Dick HM, Carmichael DJ. Reconstituted antigen-poor collagen preparations as potential pulp-capping agents. Journal of Endodontics. 6(7) , 641-644 (1980) .
  5. Nevins AJ, La Porta RF, Borden BG, Spangberg LS. Pulpotomy and partial pulpectomy procedures in monkey teeth using cross-linked collagen-calcium phosphate gel. Oral Surgery, Oral Medicine and Oral Pathology. 49(4) , 360-365 (1980) .
  6. Nakashima M. Induction of dentine in amputated pulp of dogs by recombinant human bone morphogenetic proteins-2 and -4 with collagen matrix. Archives of Oral Biology. 39(12) , 1085-1089 (1994) .
  7. Jepsen S, Albers HK, Fleiner B, Tucker M, Rueger D. Recombinant human osteogenic protein-1 induces dentin formation: an experimental study in miniature swine. Journal of Endodontics. 23(6) , 378-382 (1997) .
  8. Melin M, Joffre-Romeas A, Farges JC, Couble ML, Magloire H, Bleicher F. Effects of TGFa1 on dental pulp cells in cultured human tooth slices. Journal of Dental Research. 79(9) , 1689-1696 (2000) .
  9. Pachence JM. Collagen-based devices for soft tissue repair. Journal of Biomedical Materials Research. 33(1) , 35-40 (1996) .
  10. Delbalso AM, Adrian JC. Collagen gel in osseous defects: a preliminary study. Oral Surgery, Oral Medicine and Oral Pathology. 42(5) , 562-569 (1976) .
  11. Mitchell R. An evaluation of bone healing in cavities in the jaws implanted with a collagen matrix. The British Journal of Oral & Maxillofacial Surgery. 30(3) , 180-182 (1992) .
  12. Rocha LB, Goissis G, Rossi MA. Biocompatibility of anionic collagen matrix as scaffold for bone healing. Biomaterials. 23(2) , 449-456 (2002) .
  13. DeLustro F, Smith ST, Sundsmo J, Salem G, Kincaid S, Ellingsworth L. Reaction to injectable collagen: results in animal model and clinical use. Plastic and Reconstructive Surgery. 79(4) , 581-594 (1987) .
  14. Kamer FM, Churukian MM. Clinical use of injectable collagen: a three year retrospective review. Archives of Otolaryngology. 110(4) , 93-98 (1984) .
  15. Watson SP. Collagen receptor signalling in platelets and megakaryocytes. Thrombosis and haemostasis. 82(2) , 365-76 (1999) .
  16. Chiang TM. Collagen-platelet interaction: platelet non-integrin receptors. Histology and Histopathology. 14(2) , 579-585 (1999) .
  17. Legeros RS. Calcium phosphate materials in restorative dentistry: a review. Advances in Dental Research. 2(1) , 164-180 (1988) .
  18. Tenhuisen KS, Martin RI, Klimkiewicz M, Brown PW. Formation and properties of a synthetic bone composite: hydroxyapatite-collagen. Journal of Biomedical Materials Research. 29(7) , 803-810 (1995) .
  19. Gabrielli MAC, Marcantonio-Junior E, Goissis G, Okamoto T, Gagrielli MFR, Vieira EH. Hydroxylapatite implants with or without collagen in the zygomatic arch of rats. Histological study. Brazilian Dental Journal. 12(1) , 9-15 (2001) .
  20. Subay RK, Asçi S. Human pulpal response to hydroxyapatite and a calcium hydroxide material as direct capping agents. Oral Surgery, Oral Medicine and Oral Pathology. 76(4) , 485-492 (1993) .
  21. Cirelli JA, Marcantonio-Junior E, Marcantonio A R, Marcantonio RAC, Lia ARCC, Goissis G, Rossa-Junior RC. Evaluation of anionic collagen membranes in the treatment of class II furcation lesions: an histometric analysis in dogs. Biomaterials. 18(18) , 1227-1234 (1997) .
  22. Kikuchi M, Itoh S, Ichinose S, Shinomiya K, Tanaka J. Self-organization mechanism in a bone-like hydroxyapatite/collagen nanocomposite synthesized in vitro and its biological reaction in vivo. Biomaterials. 22(13) , 1705-1711 (2001) .
  23. Mehlisch DR, Taylor TD, Leibold DG, Hiatt R, Waite DE, Waite PD, Laskin DM, Smith ST, Koretz MM. Evaluation of collagen/hydroxylapatite for augmenting deficient alveolar ridges: a preliminary report. Journal of Oral and Maxillofacial Surgery. 45(5) , 408-413 (1987) .
  24. Rego DM, Marcantonio-Junior E, Lia RCC, Goissis G. Evaluation of a collagen membrane in dogs periodontium. Histological analysis. Journal of Dental Research. 73(4):764 , (1994) .
  25. Gongloff RK, Montogomery CK. Experimental study of the use of collagen tubes for implantation of particulate hydroxyapatite. Journal of Oral and Maxillofacial Surgery. 43(11) , 845-849 (1985) .
  26. Bet MR, Goissis G, Lacerda CA. Characterization of polyanionic collagen prepared by selective hydrolysis of asparagine and glutamine carboxyamide side chains. Biomacromolecules. 2(4) , 1074-1079 (2001) .
  27. Rocha LB, Brochi MAC, Bellucci AD, Rossi MA. Efficacy of polyanionic collagen matrices for bone defect healing. Journal of Biomedical Materials Research. Part B, Applied Biomaterials. 71(2) , 355-359 (2004) .
  28. Jarcho M, Bolen CH, Thomas MB, Bobick J, Kay JF, Doremus RH. Hydroxyapatite synthesis and characterization in dense polycrystalline form. Journal of Materials Science. Materials in Medicine. 11(11) , 2027-2035 (1976) .
  29. Martins VCA, Goissis G, Ribeiro AC, Marcantonio-Júnior E, Bet MR. The controlled release of antibiotic by hydroxyapatite: anionic collagen composites. Artificial Organs. 22(3) , 215-221 (1998) .
  30. Albee FH. Studies in bone growth. Triple calcium phosphate as a stimulus to osteogenesis. Annals of Surgery. 71 , 32-36 (1920) .
  31. Alberius P. Role of osteopromotion in experimental bone grafting to the skull: a study in adult rats using a membrane technique. Journal of Oral and Maxillofacial Surgery. 50(8) , 829-834 (1992) .
  32. Anselme K. Osteoblast adhesion on biomaterials. Biomaterials. 21(7) , 667-681 (2000) .
  33. Rangel S, Leonardo MR, Silva LAB, Assed S, Nelson-Filho P. Evaluacion clinica y radiografica de dientes deciduos sometidos a la tecnica de pulpotomia hidroxido de calcio. Vida y Salud. 5(2) , 18-21 (1998) .
  34. Watts A, Paterson RC. Pulpal response to a zinc oxide-eugenol cement. International Endodontic Journal. 20(2) , 82-86 (1987) .
  35. Holland R, Mello W, Souza V, Nery MJ, Bernabé PF, Otoboni FJA. The influence of the sealing material in the healing process of inflammed pulps capped with calcium hydroxide or zinc oxide-eugenol cement. Acta de Odontologia Pediatrica. 2(1) , 5-9 (1981) .
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