1 Pesquisa Veterinária Brasileira 2012 Vol: 32(4):. DOI: 10.1590/S0100-736X2012000400003

Avaliação in vitro de clorexidina, amônia quaternária e ácido peracético frente a amostras de Salmonella Heidelberg isoladas de abatedouro avícola em 2005 e 2009

The objective of this investigation was to evaluate the in vitro sensibility of Salmonella Heidelberg to three commercially available disinfectants used for sanitization in poultry slaughterhouses. A total of 20 S. Heidelberg were tested (14 isolated in 2005 and six in 2009), and as active ingredients were used chlorhexidine (0.5%), quaternary ammonium (0.5 %) and peracetic acid (1%) at contact intervals of 5, 10, 15 e 20 minutes. All isolates were found to be sensitive to peracetic acid at four specific contact intervals. One hundred percent of S. Heidelberg isolated in 2005 was found to be sensitive to quaternary ammonium, while 33% of 2009 isolates were resistant at a 5-minute contact interval and 16.6% at 10-minutes. With respect to chlorhexidine, 25% of the 2005 isolates were resistant at a 5-minute contact interval, 33% of the 2009 isolates were resistant with the same time, and 17% at a 10-minute contact interval. It can be concluded that the highest disinfectant activity in vitro was found to be with peracetic acid for S. Heidelberg isolates in 2005 and 2009, whereas chlorhexidine and quaternary ammonium had a reduced action against 2009 isolates, indicating the progression of bacterial resistance against these sanitizers and the need for periodic evaluation and rotation of active principles for sanitization.

Mentions
Altmetric
References
  1. Bailey J.S., Cox A.C. & Blankenship L.C. A comparison of an enzime imunoassay, DNA hibridization, antibody immobilization, and conventional methods for recovery of naturally occurring Salmonellae from processed broiler carcasses. J. Food Protection 54 , 354-356 (1991) .
  2. Beltrame C.A. Avaliação da eficiência de sanitizantes utilizados pelas indústrias de alimentos. Dissertação de Mestrado em Engenharia de Alimentos, Departamento de Ciências Agrárias, Universidade Regional Integrada do Alto Uruguai e das Missões, Erechim, RS. 78p , (2009) .
  3. Borowsky L.M., Bessa M.C., Cardoso M.I. & Avancini C.A.M. Sensibilidade e resistência de amostras de Salmonella typhimurium isoladas de suínos abatidos no Rio Grande do Sul/Brasil frente aos desinfetantes químicos quaternário de amônio e iodofor. Ciência Rural 36 , 1474-1479 (2006) .
  4. Brasil Instrução Normativa nº 62 de 26 de agosto de 2003. Oficializar os métodos analíticos oficiais para análises microbiológicas para controle de produtos de origem animal e águas. Secretária de Defesa Agropecuária, MAPA. Diário Oficial da República Federativa do Brasil, Brasília, Seção 1, p.14, 18 de setembro de 2003 , (2003) .
  5. Desmidt M., Ducatelle R. & Haesebrouck F. Serological and bacteriological observations on experimental infection with Salmonella hadar in chickens. Veterinary Microbiology 60 , 259-269 (1998) .
  6. Dufrenne J., Ritmeester W. & Van Asch Quantification of the contamination of chicken and chicken products in The Netherlands with Salmonella and Campylobacter. J. Food Protection 64 , 538-541 (2001) .
  7. Frasier M.C. Microbiologia de los alimentos. Acribia, Zaragoza. 681p , (1993) .
  8. Moraes M.S.V., Andrade N.J., Chaves J.B.P., Passos F.J.V. & Gomide L.A.M. Isolament of aerobic mesofilic and thermofilic spores in equipaments of poultry slaughter and their resistance against the chemists disinfectants. Ciênc. Tecnol. Aliment.17 , 325-329 (1997) .
  9. Pardi M.C., Santos I.F., Souza E.R. & Pardi H.S. Ciência, Higiene e Tecnologia da Carne: riscos microbiológicos da carne. Vol.1. UFG, Goiânia , 294-308 (1995) .
  10. Pelczar M. Microbiologia. Vol.1. McGraw-Hill do Brasil, São Paulo , (1980) .
  11. Romão C.M.C.A. Desinfecção e esterilização química, p. In: Teixeira P. (Ed.), Biossegurança: uma abordagem multidisciplinar. Fiocruz, Rio de Janeiro , 133-162 (1996) .
  12. Sander J.E., Hofacre C.L., Cheng I.H. & Wyatt R.D. Investigation of resistance of bacteria from commercial poultry sources to commercial disinfectants. Avian Dis. 46 , 997-1000 (2002) .
  13. Sundheim S., Langsrud E.H. & Holck A.L. Bacterial resistance to disinfectants containing quaternary ammonium compounds. I. Biodeterioration and Biodegradation 41 , 235-239 (1998) .
Expand