1 Revista da Sociedade Brasileira de Medicina Tropical 2003 Vol: 36(4):. DOI: 10.1590/S0037-86822003000400013

Freqüência de soropositividade para antígenos de Toxocara canis em crianças de classes sociais diferentes

Frequency of seropositivity for Toxocara in children from different socioeconomic strata in the city of Brasilia (Brazil) was measured. Six hundred and two children of both sexes, aged one to 12 years were distributed in two socioeconomically distinct groups. The samples of sera of the first group were obtained from blood drawn for routine tests in the laboratory of a public hospital attending children from low-income families. Samples from the second group were obtained from private laboratories attending children from middle-class families. Anti-toxocara antibodies were detected by ELISA, using Toxocara canis excretory-secretory antigens previously absorbed with Ascaris suum extract. The prevalence of seropositivity was 21.8% (66/302) in the first group and 3% (9/300) in the second (p< 0.0001). No differences in frequency according to age or sex could be detected. Our results suggest a high prevalence of childhood toxocariasis in Brasilia, with children from lower income brackets being the most affected.

  1. Ajayi OO, Duhlinska DD, Agwale SM, Njoku M. Frequency of human toxocariasis in Jos, Plateau State, Nigeria. Memórias do Instituto Oswaldo Cruz 95 , 147-149 (2000) .
  2. Alonso JM, Bojanich MV, Chamorro M, Gorodner JO. Toxocara seroprevalence in children from a subtropical city in Argentina. Revista do Instituto de Medicina Tropical de São Paulo 42 , 235-237 (2000) .
  3. Baboolal S, Rawlins SC. Seroprevalence of toxocariasis in schoolchildren in Trinidad. Transaction Royal Tropical Medicine and Hygiene 96 , 139-143 (2002) .
  4. Beaver PC, Snyder H. Carrera G. Chronic eosinophilia due to visceral larva migrans: report of three cases. Pediatrics 9 , 7-19 (1952) .
  5. Cilla G, Perez-Trallero E, Gutierrez C, Part C, Gomariz M. Seroprevalence of Toxocara infection in middle-class and disadvantaged children in northern Spain (Gipuzkoa, Basque Cauntry). European Journal of Epidemiology 12 , 541-543 (1996) .
  6. De Savigny DH. In vitro maintenance of Toxocara canis larvae and a simple method for the production of Toxocara ES antigen for use in serodiagnostic tests for visceral larva migrans. Journal of Parasitology 61 , 781-782 (1975) .
  7. De Savigny DH, Voller A. ELISA for toxocariasis using larval secretory antigens. Transaction Royal Society of Tropical Medicine and Hygiene 73 , 106-115 (1978) .
  8. De Savigny DH, Voller A, Woodruff AW, Toxocariasis: serological diagnosis by enzyme immunoassay. Journal of Clinical Pathology 32 , 284-288 (1979) .
  9. Elefant GR, Jacob CMA, Kanashiro EHY, Peres, BA. Toxocaríase. In: Ferreira AW, Ávila SLM (eds) Diagnóstico laboratorial das Principais doenças infecciosas e auto-imunes. 2ª edição Guanabara Koogan. Rio de Janeiro , 323-332 (2001) .
  10. Gueglio B, de Gentile L, Nguyen JM, Achard J, Chabasse D, Marjolet M. Epidemiological approach to human toxocariasis in westwern France. Parasitology Research 80 , 531-536 (1994) .
  11. Kanamura HY, Hoshino-Shimizu S, Silva LC. Solubilization of antigen S. mansoni adult worms for the passive hemagglutination test. Revista do Instituto de Medicina Tropical de São Paulo 23 , 92-95 (1981) .
  12. Lowry VH, Rosembrouch NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193 , 265-271 (1956) .
  13. Lynch NR, Hagel I, Vargas V, Rotundo A, Varela MC, Di prisco MC, Hodgen AN. Comparable seropositivity for ascariasis and toxocariasis in tropical slum children. Parasitololgy Research 79 , 547-550 (1993) .
  14. Nunes CM, Tundisi RN, Garcia JF, Heinemann MB, Ogassawara S, Richtzenhain LJ. Cross-reaction between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique. Revista do Instituto de Medicina Tropical de São Paulo 39 , 1-8 (1997) .
  15. Nunes CM, Tundisi RN, Heinemann MB, Ogassawara S, Richtzenhain LJ. Toxocariasis: serological diagnosis by indirect antibody competition ELISA. Revista do Instituto de Medicina Tropical de São Paulo 41 , 95-100 (1999) .
  16. Radman NE, Archelli SM, Fonrouge RD, del V Guardis M, Linzitto OR. Human toxocariasis. Its seroprevalence in the city of La Plata. Memórias do Instituto Oswaldo Cruz 95 , 281-285 (2000) .
  17. Shields JA. Ocular toxocariasis. A review. Survey of Ophtalmolology 28 , 361-381 (1984) .
  18. Silva FM, Leão ME, Mendonça HFS, Pereira FEL. Prevalence of anti-Toxocara antibodies in a random sample of impatients at a children's hospital in Vitória, Espírito Santo, Brazil. Revista do Instituto de Medicina Tropical de São Paulo 40 , 1-6 (1998) .
  19. Yamasaki H, Araki K, Chooi Lim PK, Zasmy N, Wah Mak J, Taib R, Aoki T. Development of a highly specific recombinant Toxocara canis second-stage larva excretory-secretory antigen for immunodiagnosis of human toxocariasis. Journal of Clinical Microbiology 38 , 1409-1413 (2000) .