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Genome constitution of Narcissus variety, ‘Tête-à-Tête’, analysed through GISH and NBS profiling

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Abstract

The Narcissus variety, ‘Tête-à-Tête’, has been the most popular variety since 1949, and a well known allotriploid (2n = 3x = 24 + B) of spontaneous origin. Because the identity of one of the parents of this variety was uncertain, the genome constitution of ‘Tête-à-Tête’ was investigated by using genomic in situ hybridization (GISH) and NBS profiling. Both of these techniques confirmed that two different species of Narcissus, viz., N. tazetta (2n = 2x = 20) and N. cyclamineus (2n = 2x = 14) are the parents. GISH clearly identified 10 chromosomes of N. tazetta and 14 chromosomes of N. cyclamineus, the former has contributed one and the latter has contributed two genomes. One B chromosome was identically labelled as those of N. cyclamineus indicating the affinity of the special chromosome to this species. Due to its male and female sterility ‘Tête-à-Tête’ is unsuitable as a parent for utilizing in further breeding, it might be possible to re-synthesize a ‘Tête-à-Tête’ like variety using the now known parents and the original pathway.

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Authors and Affiliations

  1. College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China

    Hongzhi Wu

  2. Plant Breeding, Wageningen University and Research Center, Wageningen, The Netherlands

    Hongzhi Wu, Munikote S. Ramanna, Paul Arens & Jaap M. van Tuyl

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  1. Hongzhi Wu
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  2. Munikote S. Ramanna
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  3. Paul Arens
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  4. Jaap M. van Tuyl
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Correspondence to Hongzhi Wu or Jaap M. van Tuyl.

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Wu, H., Ramanna, M.S., Arens, P. et al. Genome constitution of Narcissus variety, ‘Tête-à-Tête’, analysed through GISH and NBS profiling. Euphytica 181, 285–292 (2011). https://doi.org/10.1007/s10681-011-0438-6

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  • DOI: https://doi.org/10.1007/s10681-011-0438-6

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