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The temperature response of CO2 assimilation, photochemical activities and Rubisco activation in Camelina sativa, a potential bioenergy crop with limited capacity for acclimation to heat stress

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Abstract

The temperature optimum of photosynthesis coincides with the average daytime temperature in a species’ native environment. Moderate heat stress occurs when temperatures exceed the optimum, inhibiting photosynthesis and decreasing productivity. In the present study, the temperature response of photosynthesis and the potential for heat acclimation was evaluated for Camelina sativa, a bioenergy crop. The temperature optimum of net CO2 assimilation rate (A) under atmospheric conditions was 30–32 °C and was only slightly higher under non-photorespiratory conditions. The activation state of Rubisco was closely correlated with A at supra-optimal temperatures, exhibiting a parallel decrease with increasing leaf temperature. At both control and elevated temperatures, the modeled response of A to intercellular CO2 concentration was consistent with Rubisco limiting A at ambient CO2. Rubisco activation and photochemical activities were affected by moderate heat stress at lower temperatures in camelina than in the warm-adapted species cotton and tobacco. Growth under conditions that imposed a daily interval of moderate heat stress caused a 63 % reduction in camelina seed yield. Levels of cpn60 protein were elevated under the higher growth temperature, but acclimation of photosynthesis was minimal. Inactivation of Rubisco in camelina at temperatures above 35 °C was consistent with the temperature response of Rubisco activase activity and indicated that Rubisco activase was a prime target of inhibition by moderate heat stress in camelina. That photosynthesis exhibited no acclimation to moderate heat stress will likely impact the development of camelina and other cool season Brassicaceae as sources of bioenergy in a warmer world.

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Notes

  1. Mention of a trademark, proprietary product or vendor does not constitute a guarantee or warranty of the product by the United States Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that may also be suitable.

Abbreviations

A :

Rate of net CO2 assimilation

A c :

Rubisco-limited rate of CO2 assimilation

A j :

Electron-transport-limited rate of CO2 assimilation

C i :

Intercellular CO2 concentration

C trans :

Intercellular CO2 concentration at which A c and A j are co-limiting

F o :

Minimal chlorophyll fluorescence of a dark-adapted leaf

F v/F m :

Maximum quantum efficiency of photosystem II

ϕPSII :

Operating efficiency of photosystem II

k cat :

Turnover number

NPQ:

Non-photochemical quenching

J :

Maximum rate of electron transport

PPFD:

Photosynthetic photon flux density

PSII:

Photosystem II

Rubisco:

Ribulose-1,5-bisphosphate carboxylase/oxygenase

V cmax :

Maximum velocity of RuBP carboxylation

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Acknowledgments

The authors acknowledge the expert technical assistance of Nancy Parks and Heather Malone (USDA-ARS, Maricopa, Arizona, USA). This work was funded by the Division of Chemical Sciences, Geosciences, and Biosciences, Office of Basic Energy Sciences, of the United States Department of Energy through Grant DE-FG02-10ER20268 to M.E.S..

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  1. US Department of Agriculture, Agricultural Research Service, Arid-Land Agricultural Research Center, Maricopa, AZ, 85138, USA

    A. Elizabete Carmo-Silva & Michael E. Salvucci

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  1. A. Elizabete Carmo-Silva
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  2. Michael E. Salvucci
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Correspondence to Michael E. Salvucci.

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Carmo-Silva, A.E., Salvucci, M.E. The temperature response of CO2 assimilation, photochemical activities and Rubisco activation in Camelina sativa, a potential bioenergy crop with limited capacity for acclimation to heat stress. Planta 236, 1433–1445 (2012). https://doi.org/10.1007/s00425-012-1691-1

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